Journal: BBA Clinical

Article Title: Transferrin-bound proteins as potential biomarkers for advanced breast cancer patients

doi: 10.1016/j.bbacli.2014.08.004

Figure Lengend Snippet: List of proteins found to be differentially expressed at statistically significant levels (p ≤ 0.05, fold change ≥ 2) when comparing Transferrin-associated proteins in normal control (n = 10) to stage IV breast cancer (n = 10) patient serum samples (Discovery sample set). Included in the table is the gene symbol count, c, protein identification, peptide confidence score (Mascot), p-value, fold-change and highest/lowest mean change.

Article Snippet: ELISA kits for Fibrinogen (EF1040-1) and for Fibronectin (EF1045-1) from Assaypro were used as per manufacturer's instructions (AssayPro, Winfield, MO, USA).

Techniques:

Journal: BBA Clinical

Article Title: Transferrin-bound proteins as potential biomarkers for advanced breast cancer patients

doi: 10.1016/j.bbacli.2014.08.004

Figure Lengend Snippet: Box and whisker plots (range, median, quartiles) of A. Fibronectin, B. Fibrinogen and C. CA15-3 in control, IBD (Inflammatory Bowel Disease), benign breast disease and stages I–IV breast cancer patient serum samples (validation sample set). Fibrinogen, Fibronectin and CA15-3 were all found to be statistically significant when comparing abundance levels between normal control and stage IV breast cancer patient serum samples. Both Fibrinogen and CA15-3 were also statistically significant comparing IBD or benign breast disease to stage IV breast cancer. Samples: normal healthy control (n = 54), inflammatory bowel disease (n = 19), benign breast disease (n = 12), stage I breast cancer (n = 20), stage II breast cancer (n = 20), stage III breast cancer (n = 39) and stage IV breast cancer (n = 76).

Article Snippet: ELISA kits for Fibrinogen (EF1040-1) and for Fibronectin (EF1045-1) from Assaypro were used as per manufacturer's instructions (AssayPro, Winfield, MO, USA).

Techniques: Whisker Assay

Journal: Journal of Assisted Reproduction and Genetics

Article Title: GRN, NOTCH3, FN1, and PINK1 expression in eutopic endometrium – potential biomarkers in the detection of endometriosis – a pilot study

doi: 10.1007/s10815-020-01905-4

Figure Lengend Snippet: Dataset of conducted experiments with some descriptive statistics showing the sample size, median, range , and standard deviation

Article Snippet: The following kits were used: Progranulin ELISA Kit (No. E-EL-H1578, Elabscience, USA), Human Notch Homolog 3 ELISA Kit (No. SEL147Hu, Cloud-Clone Corp., USA), Quantikine ELISA Human Fibronectin (No. DFBN10, R&D Systems, USA), Human PINK1 ELISA Kit (No. MBS9327222, MyBioSource, USA).

Techniques: Standard Deviation, Control, Enzyme-linked Immunosorbent Assay

Journal: Journal of Assisted Reproduction and Genetics

Article Title: GRN, NOTCH3, FN1, and PINK1 expression in eutopic endometrium – potential biomarkers in the detection of endometriosis – a pilot study

doi: 10.1007/s10815-020-01905-4

Figure Lengend Snippet: Changes in protein expression levels. Results of enzyme-linked immunosorbent assay (ELISA) of human eutopic endometrial tissue. Relative protein expression levels of GRN and FN1 to the total protein amount of the endometrial tissue sample. Bar graphs showing protein amount in pg (GRN) or ng (FN1) per 1 mg of total protein amount. Data represents the mean ± SEM. Control (without EM, n = 10); ASRM I (minimal EM, n = 10); ASRM II (mild EM, n = 10); ASRM III (moderate EM, n = 10). * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: The following kits were used: Progranulin ELISA Kit (No. E-EL-H1578, Elabscience, USA), Human Notch Homolog 3 ELISA Kit (No. SEL147Hu, Cloud-Clone Corp., USA), Quantikine ELISA Human Fibronectin (No. DFBN10, R&D Systems, USA), Human PINK1 ELISA Kit (No. MBS9327222, MyBioSource, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Translational Vision Science & Technology

Article Title: Tissue Architecture Modulates Compositional and Structural Properties of Corneal Myofibroblast-Derived Matrix

doi: 10.1167/tvst.14.9.9

Figure Lengend Snippet: ( A ) Fibronectin deposition ( green ) and CHP ( magenta ) in aligned and random constructs at day14 in Z-stack images from whole mounts obtained with a confocal microscope. Scale bars : 20 µm. ( B – D ) ELISA assays were used to evaluate the protein concentration in relation to total protein content of the following proteins: fibronectin ( B ), decorin ( C ), and tenascin C ( D ). N = 32, derived from four donors, and each N represents the content from two construct lysates pooled together. Data presented as mean ± SD. ** P < 0.01; *** P < 0.001; **** P < 0.001; t P < 0.05; ### P < 0.001; #### P < 0.001; #, main effect of construct type.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used for detection of the following soluble protein fractions: collagen type I ( EKC33223 ; Biomatik, Kitchener, ON, Canada), collagen type V ( EKU11733 ; Biomatik), fibronectin (DFBN10; R&D Systems, Minneapolis, MN), tenascin C (EH446RB; Thermo Fisher Scientific), and decorin (Thermo Fisher Scientific) according to each manufacturer's instructions.

Techniques: Construct, Microscopy, Enzyme-linked Immunosorbent Assay, Protein Concentration, Derivative Assay

Journal: Chinese Medical Journal

Article Title: Exosomal miR-485-3p derived from pancreatic ductal epithelial cells inhibits pancreatic cancer metastasis through targeting PAK1

doi: 10.1097/cm9.0000000000002154

Figure Lengend Snippet: Figure 2: MiR-485-3p regulated PC cell migration and Invasion. (A, B) miR-485-3p was overexpressed and silenced In BxPC-3 and PANC-1 cells. (C, D) Transwell migration and Invasion assays in BxPC-3 and PANC-1 cells with overexpression or silencing of miR-485-3p (bar: 100 mm). The migrated or invaded cells were imaged at 200 magnification in five random fields and counted using ImageJ software. (E) MiR-485-3p was overexpressed and silenced in PSCs. (F) FN1, COLIA1, and FAP levels in cell supernatant of PSCs transfected with miR-485-3p mimic or Inh-miR-485-3p were assessed by ELISA. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. COLIA1: Collagen type alpha 1 chain; ELISA: Enzyme linked immunosorbent assay; FAP: Fibroblast activation protein alpha; FN1: Fibronectin 1; PC: Pancreatic cancer; PSCs: Pancreatic stellate cells.

Article Snippet: Enzyme linked immunosorbent assay (ELISA) The levels of fibronectin 1 (FN1), Col I, and fibroblast activation protein alpha (FAP) in PSCs cell supernatant were detected by using Human FN ELISA Kit (No. E-ELH0179c, Elabscience, Wuhan, China), Human Col I ELISAKit (No. JM-03329H1, JINGMEI, Jiangsu, China), and Human FAPa ELISA Kit (No. EH3037, FineTest, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Migration, Over Expression, Software, Transfection, Enzyme-linked Immunosorbent Assay, Activation Assay

Journal: Biomedicines

Article Title: Niclosamide Attenuates Inflammation-Associated Profibrotic Responses in Human Subepithelial Lung Myofibroblasts.

doi: 10.3390/biomedicines11072032

Figure Lengend Snippet: Figure 1. The effect of niclosamide on the IL-1α and TNF-α-inducible mRNA expression of collagen type I, collagen type III, and fibronectin. Collagen type I (A), collagen type III (B), and fibronectin (C) mRNA expression levels in SELMs after stimulation with IL-1α, TNF-α or their combination, treated or not with niclosamide at 30 nM and 100 nM concentrations. Mean values of data from experiments per- formed in triplicates on SELMs from 4 individuals are shown. 2C: IL-1α + TNF-α, N30: niclosamide 30 nM, N100: niclosamide 100 nM.

Article Snippet: Human fibronectin was measured in SELM culture supernatants using a commercially available kit (human fibronectin enzyme-linked immunosorbent assay [ELISA] kit, Origene, Biomedicines 2023, 11, 2032 5 of 14 Rockville, MD, USA) as previously described [27] and according to the manufacturer’s instructions.

Techniques: Expressing

Journal: Biomedicines

Article Title: Niclosamide Attenuates Inflammation-Associated Profibrotic Responses in Human Subepithelial Lung Myofibroblasts.

doi: 10.3390/biomedicines11072032

Figure Lengend Snippet: Figure 2. The effect of niclosamide on the TGF-β1-inducible expression of collagen type I, collagen type III, and fibronectin. Collagen type I (A), collagen type III (B), and fibronectin (D) mRNA expression levels in SELMs after stimulation with TGF-β1 with or without treatment with niclosamide at 100 nM concentration. Total secreted collagen from SELMs stimulated with TGF-β1 with or without treatment with niclosamide at 100 nM concentration (C). Mean values of data from experiments performed in triplicate on SELMs from 4 individuals (A,B,D) and 3 individuals (C) are shown. N100: niclosamide 100 nM.

Article Snippet: Human fibronectin was measured in SELM culture supernatants using a commercially available kit (human fibronectin enzyme-linked immunosorbent assay [ELISA] kit, Origene, Biomedicines 2023, 11, 2032 5 of 14 Rockville, MD, USA) as previously described [27] and according to the manufacturer’s instructions.

Techniques: Expressing, Concentration Assay

Journal: Molecules

Article Title: Effects of the Calix[4]arene Derivative Compound OTX008 on High Glucose-Stimulated ARPE-19 Cells: Focus on Galectin-1/TGF-β/EMT Pathway

doi: 10.3390/molecules27154785

Figure Lengend Snippet: EMT markers in ARPE-19 cells. ( A ) ACTA2 and ( B ) Fn1 mRNA and protein levels. ACTA2 and Fn1 relative mRNA levels are reported as 2 ^−ΔΔCt ± SD and quantized by using GAPDH as control. ACTA2 and Fn1 protein levels are reported as ng/mL ± SD of 9 observations per group (three independent experiments, each done in triplicate). ** p < 0.01 vs. NG; ° p < 0.05 and °° p < 0.01 vs. HG; NG, normal glucose (5 mM) + vehicle; M, mannitol (30 mM); HG, high glucose (35 mM) + vehicle; OTX 2.5, OTX 2.5 µM; OTX 5, OTX 5 µM; OTX 10, OTX 10 µM.

Article Snippet: Competitive ELISA tests were used to quantify the cellular levels of human AP4S1 (My Biosource, MBS7206462), ACTA2 (or α-SMA) (antibodies-online, ABIN6953406), and Fn1 (Cusabio, CSB-E11850h).

Techniques: Control

Journal: Journal of Translational Medicine

Article Title: LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation

doi: 10.1186/s12967-020-02310-2

Figure Lengend Snippet: LTBP1 contributed to CAFs transformation and expression of FN1 induced by ESCC cells. a The expression of LTBP1 and FN1 were positively correlated in the GEPIA database (Spearman correlation analysis, r = 0.6, p < 0.001). b , c The expression of LTBP1 and FN1 were detected in various cells by western blot and ELISA. d , f Western blot and immunofluorescent analysis of α-SMA and FN1 expression in fibroblasts which were con-cultured with si-NC, si-LTBP1 ESCC cells or negative for 96 h. e CCK8 assays comparing the effect of (± siLTBP1 ESCC cells or negative) conditioned medium on the activity of the fibroblasts. Each experiment was performed in triplicate. Paired-samples t-test, *p < 0.05

Article Snippet: The levels of FN1 in conditioned medium of various cells were measured using Human Fibronectin ELISA Kit (CSB-E04551h; CUSABIO) according to the manufacturer’s instructions.

Techniques: Transformation Assay, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture, Activity Assay

Journal: Cancers

Article Title: ROCK2 Confers Acquired Gemcitabine Resistance in Pancreatic Cancer Cells by Upregulating Transcription Factor ZEB1

doi: 10.3390/cancers11121881

Figure Lengend Snippet: Primer for PCR assay.

Article Snippet: Fibronectin was purchased from Proteintech Group Inc. P-ROCK1 (Thr455 + Ser456) was purchased from Bioss (Beijing, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Effective Restoration of Gastric and Esophageal Tissues in an In Vitro Model of GERD: Mucoadhesive and Protective Properties of Xyloglucan, Pea Proteins, and Polyacrylic Acid

doi: 10.3390/ijms26094409

Figure Lengend Snippet: Evaluation of barrier integrity of GTL-16 cells. ( A ) TEER (transepithelial electrical resistance) value measured using EVOM3 at different timepoints (from 1 h to 4 h); ( B – D ) analysis of TJ measured by Enzyme-Linked Immunosorbent Assay (ELISA) test (Claudin-1, ZO-1, and occludin, respectively) at 4 h. HCl = positive control, cells in GERD condition induced by HCl; Control = negative control, untreated cells. Data are expressed as means ± SD (%) of 5 independent experiments. * p < 0.05 vs. negative control; φ p < 0.05 vs. positive control. HCl: hydrochloric acid; TEER: trans-epithelial electrical resistance; XXPA: combination of xyloglucan, pea protein, and polyacrylic acid; ZO-1: Zonula Occludens-1.

Article Snippet: Fibronectin quantification was determined using the Human FN1 (Fibronectin) ELISA Kit (FineTest, Wuhan, China) according to the manufacturer’s instructions on GTL-16 cell lysates [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Control, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Effective Restoration of Gastric and Esophageal Tissues in an In Vitro Model of GERD: Mucoadhesive and Protective Properties of Xyloglucan, Pea Proteins, and Polyacrylic Acid

doi: 10.3390/ijms26094409

Figure Lengend Snippet: Adhesion study on GTL-16 cells. ( A ) Vitronectin and ( B ) fibronectin analysis measured by Enzyme-Linked Immunosorbent Assay (ELISA) test at 4 h. HCl = positive control, cells in GERD condition induced by HCl; Control = negative control, untreated cells. Data are expressed as means ± SD (%) of 5 independent experiments. * p < 0.05 vs. negative control; φ p < 0.05 vs. positive control. HCl: hydrochloric acid; XXPA: combination of xyloglucan, pea protein, and polyacrylic acid.

Article Snippet: Fibronectin quantification was determined using the Human FN1 (Fibronectin) ELISA Kit (FineTest, Wuhan, China) according to the manufacturer’s instructions on GTL-16 cell lysates [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Control, Negative Control